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August 2014 Closures Issue

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p r a c t i c a l w i n e r y & v i n e ya r d a U G U S t 2 0 1 4 69 sotolon and phenyl acetaldehyde. 9 The addition of pure glutathione to fin- ished wine, however, is not legally per- mitted. There are several yeast-derived products available that could increase a wine's glutathione level. These yeast- derived products are rich in glutathione and the precursors for glutathione pro- duction including cysteine and N-acetyl- cysteine. The potential to enhance the final wine depends on the original gluta- thione content and composition of the wine after primary fermentation and handling of the wine from a redox per- spective. Addition of these products as nutri- tional aids during the first third of pri- mary fermentation are legal and assist the yeast internal redox balance and enhances the final glutathione content of wines and, as a result, increases the sta- bility of the volatile thiols and thus the longevity of a wine. Autolyzed yeast nutritional aids are allowed by TTB Reg- ulation 27 CFR 24.246 —Materials autho- rized for the treatment of wine and juice. Conclusions Winemaking consists of a series of steps. Each step requires a calculated decision by the winemaker. A good winemaker makes informed decisions and takes the neces- sary precautions to protect and possibly enhance the quality delivered to him/her from the vineyard. Various winemaking practices influence white wine aroma. In the pursuit of quality aromatic white wines, decisions regarding the extraction of positive flavor-aroma character from the incoming grapes must be balanced with the possibility of over extraction and obtaining undesirable components. Greater extrac- tion of the must by several mechanisms— including proper enzyme selection and use, followed by removal of excess phenolics by selective juice fining, can allow for more positive flavor-aroma compounds. Enhancement of the flavor-aroma char- acter through proper yeast strain selec- tion, rehydration product addition and fermentation nutrition protocols can add significantly to the flavor-aroma profile of a white aromatic wine in both com- plexity and volume. The extraction and enhancement of w h i t e a ro m a t i c w i n e f l a v o r- a ro m a must be followed with a strategy for preservation of the gains accomplished t h ro u g h p ro p e r d e c i s i o n m a k i n g . Many processing decisions made by the winemaker will impact the delicate flavor-aroma compounds and oxida- tion is the true enemy of these critical molecules. Understanding oxidation-reduction reactions that can occur in wine and the treatments available to assist in anti-oxi- dation are keys to making expressive aro- matic white wines that will deliver their potential to the wine enthusiast upon consumption. PWV This text was edited from the October 2013 Australia & New Zealand Grapegrower & Winemaker with permission of the pub- lisher, Winetitles. Bibliography 1. Blateyron, L., and J.M. Sablayrolles. 2001 "Stuck and slow fermentations in enology: statistical study of causes and effectiveness of combined addi- tions of oxygen and diammonium phosphate." J. Biosci. Bioeng. 91,184–189. 2. Canal-Llaubéres, R-M. 2010 "Enzymes and wine quality." In: Managing Wine Quality Vol. 2, 93–132. 3. Chatonnet, P., C. Barbe, R. Canal- Llaubères, D. Dubourdieu and J-N Boidron. 1992 "Incidence de certaines préparations pectolytiques sur la teneur en phénols volatils des vins blancs." J. Int. Sci. Vigne Vin, 26 (4), 253–269. 4. Cordente, A.G., C.D. Curtin, C. Varela and I.S. Pretorius. 2012 "Flavour active wine yeasts." Appl. Microbiol. Biotechnol. 96, 601–618. 5. Curtin, C.D., J.R. Bellon, E.J. Bartowsky, P.A. Henschke., P.J. Chambers, M.J. Herderich and I.S. Pretorius. 2011 "Harnessing AWRI's yeast and bacterial research to shape 'Next-Gen' Chardonnay Part 2: Influence of yeast, nutritional manage- ment and malolactic fermentation." Wine & Viticulture J. 26 (2), 15–24. 6. Gambuti, A., A. Rinaldi and L. Moio. 2012 "Use of patatin, a protein extracted form potato, as alternative to animal proteins in fining of red wine." Eur. Food Res. Technol. DOI 10.1007/ s00217-012-1791-y. 7. Henschke, P.A., and V. Jiranek. 1993 "Yeasts—metabolism of nitrogen compounds." In: Fleet GH (ed) Wine Microbiology and Biotechnology, Harwood Academic Publishers, Chur, Switzerland, 77–164. 8. Howell, K.S., J.H. Swiegers, G.M. Elsey, T.E. Siebert, E.J. Bartowsky, G.H. Fleet, I.S. Pretorius and M.A. De Barros Lopes. 2004 "Variation in 4-mercapto-4-methyl-pentan-2-one release by Saccharomyces cerevisiae commercial wine strains." FEMS Microbiol. Lett. 240, 125–129. 9. Lavigne, V., A. Pons and D. Dubordieu. 2007 "Assay of glutathi- one in must and wines using capillary electrophoresis and laser-induced fluorescence detection: Changes in concentration in dry white wines dur- ing alcoholic fermentation and aging." J. Chromatography A, 1139 (1), 130–135. 10. Marullo, P. "Yeast selection for wine flavour modulation." 2010 In: Managing Wine Quality Vol. 2, 293–345. 11. Moine, V., M.L. Murat, C. Arfeuillère and C. Thibon. 2012 "Collage des jus de presse blanc—Influence sur leurs teneurs en composés phénoliques en glutathion et précurseur d'arômes." Revue des œnologues, 139, 45–47. 12. Oelofse, A., I.S. Pretorius and M. Du Toit. 2008 "Significance of Brettanomyces and Dekkera during winemaking: A Synoptic Review." S. Afr. J. Enol. Vitic., Vol. 29, No. 2, 128–144. 13. O'Kennedy, K. and G.C. Reid. 2008 "Yeast Nutrient Management." Aus. & NZ Grapegrower & Winemaker, 537, 92–100. 14. Peyrot des Gachons, C., T. Tominaga and D. Dubourdieu. 2002 "Sulfur aroma precursor present in S-glutathione conjugate form: indenti- fication of S-3-(Hexan-1-ol)-glutathione in must from Vitis Vinifera L. cv. Sauvignon blanc." J. Agric. Food Chem. 50, 4076–4079. 15. Sumby, K.M., P.R. Grbin and V. Jiranek. 2010 "Microbial modulation of aromatic esters in wine: current knowledge and future prospects." Food Chem. 121, 1–16. 16. Winter, G., P.A. Henschke, V.J. Higgins, M. Ugliano and C. Curtin. 2011 "Effects of rehydration nutrient on H2S metabolism and formation of volatile sulphur compounds in wine." AMB Express 1:36 (1–11). W I N E M A K I N G Figure 5. A comparison of the aromatic in- dexes of volatile thiols of wines from the same must that received a glutathione/precursor product late and early in fermentation respec- tively. Analyses were done immediately after completion of alcoholic fermentation and three months later. (Aromatic index: Concentration of the aroma compound in the wine, divided by its sensory threshold.) Figure 6. A comparison of the glutathione con- tent and total reductive power of six different commercial products.

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